Gamma-Ray Technology in Secondary Metabolite Production for Sustainable Agriculture: Article Review
DOI:
https://doi.org/10.24903/ajip.v14i2.3844Keywords:
gamma ray, productiom, secondary metaboliteAbstract
The increasing worldwide demand for natural products in fields ranging from pharmaceuticals to food industry has made clear an urgent necessity of more effective and sustainable production technologies. In this regard, the gamma-ray technology is a very promising Biotechnological tool of choice. Because of its effective physical mutation and unique biological effects, gamma irradiation has been responsible for more than 60% of professionally released mutant crop verities. This approach possesses the potential to exploit the mutagenic effects of ionizing radiation, and to cause genetic diversity in plants for their agronomic traits such as yield productivity and specifically at large scale, the enhancement of important secondary metabolite profiles. The utility of gamma rays goes beyond mutations induction: as an elicitor, they cause dramatic physiological and molecular responses in plants leading to the overproduction of bioactive compounds. The mode of action may be through the generation of DNA damage, e.g., single and double strand breaks, which runs a novel pathway or activates an alternative one leading to enhanced production of certain secondary metabolites. This review intended to comprehensively discuss the versatile bioprocesses of gamma irradiation, biological mechanisms underlying its action and how this effect interprets into metabolite production, crop quality and overall agricultural sustainability. The application of this technology provides a promising option not only to improve the productivity high-value natural products but also the development of new plant germplasms with enhanced resilience (or stress tolerance) that is relevant for securing food production in an era of climate change.References
Abozahra, M. S., Amin, M. A., Sarker, T. C., Abd‐ElGawad, A. M., & Aboelezz, E. (2025). Molecular, biophysical, and biochemical studies on irradiated Zea mays seeds using various sources of gamma rays for dosimetrical applications. Scientific Reports, 15(1). https://doi.org/10.1038/s41598-025-87531-5
Adabi, R., & Rezaei, A. (2023). Influence of mild γ-irradiation on growth and paclitaxel biosynthesis in hazel (Corylus avellana L.) in vitro culture. Plant Cell Tissue and Organ Culture (PCTOC), 156(1). https://doi.org/10.1007/s11240-023-02618-z
Alami, M. M., Guo, S., Mei, Z., Yang, G., & Wang, X. (2024). Environmental factors on secondary metabolism in medicinal plants: exploring accelerating factors. Medicinal Plant Biology, 3(1), 0. https://doi.org/10.48130/mpb-0024-0016
Arumingtyas, E. L., & Ahyar, A. N. (2022). Genetic diversity of chili pepper mutant ( Capsicum frutescens L.) resulted from gamma-ray radiation. IOP Conference Series: Earth and Environmental Science. https://doi.org/10.1088/1755-1315/1097/1/012059
Bharat, R. A., Prathmesh, S. P., Sarsu, F., & Suprasanna, P. (2024). Induced Mutagenesis using Gamma Rays: Biological Features and Applications in Crop Improvement. OBM Genetics, 8(2), 1. https://doi.org/10.21926/obm.genet.2402233
Cahyaningsih, A. P., Etikawati, N., & Yunus, A. (2022). Morphological characters variation of Indonesian accession Echinacea purpurea in response to gamma-ray irradiation. Biodiversitas. https://doi.org/10.13057/biodiv/d231045
Chaudhary, J., Deshmukh, R., & Sonah, H. (2019). Mutagenesis Approaches and Their Role in Crop Improvement. In Plants (Vol. 8, Issue 11, p. 467). Multidisciplinary Digital Publishing Institute. https://doi.org/10.3390/plants8110467
Damayanti, F., Roostika, I., Musliman, A., & Widodo, A. (2023). Improvement of Genetic Variation of cv Grand Naeni Bananas Through Gamma-Ray Irradiation and In Vitro Selection Using Fusaric Acid. Research Square (Research Square). https://doi.org/10.21203/rs.3.rs-3332804/v1
Devi, A. M., Devi, K. K., Devi, P. P., Devi, M. L., & Das, S. (2023). Metabolic engineering of plant secondary metabolites: prospects and its technological challenges [Review of Metabolic engineering of plant secondary metabolites: prospects and its technological challenges]. Frontiers in Plant Science, 14. Frontiers Media. https://doi.org/10.3389/fpls.2023.1171154
Du, Y., Feng, Z., Wang, J., Jin, W., Wang, Z., Guo, T., Chen, Y., Feng, H., Yu, L., Li, W., & Zhou, L. (2022). Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing in Arabidopsis thaliana. International Journal of Molecular Sciences, 23(2), 654. https://doi.org/10.3390/ijms23020654
Due, M. S., Susilowati, A., & Yunus, A. (2019). The effect of gamma rays irradiation on diversity of Musa paradisiaca var. sapientum as revealed by ISSR molecular marker. Biodiversitas. https://doi.org/10.13057/biodiv/d200534
Fadli, N., Syarif, Z., Satria, B., & Akhir, N. (2018). The Effect of Gamma Cobalt-60 Ray Irradiation on Cultivar Growth in Taro White (Xhanthosoma Sagittifolium L.). International Journal of Environment, Agriculture and Biotechnology. https://doi.org/10.22161/ijeab/3.6.9
Hanifah, W. N., Parjanto, Hartati, S., & Yunus, A. (2020). The performances of m4 generation of mentik susu rice mutants irradiated with gamma-ray. Biodiversitas. https://doi.org/10.13057/biodiv/d210915
J, J. H., & Soosairaj, S. (2024). Effect of UV B Rays on the Specific Phytochemical Synthesis in Trigonella Foenum-Graecum L. And Raphanus Sativus L. Research Square (Research Square). https://doi.org/10.21203/rs.3.rs-5546273/v1
Jain, D., Bisht, S., Parvez, A., Singh, K., Bhaskar, P., & Koubouris, G. (2024). Effective Biotic Elicitors for Augmentation of Secondary Metabolite Production in Medicinal Plants. Agriculture, 14(6), 796. https://doi.org/10.3390/agriculture14060796
Kandoudi, W., & Németh, É. (2022). Stimulating secondary compound accumulation by elicitation: Is it a realistic tool in medicinal plants in vivo? Phytochemistry Reviews, 21(6), 2007. https://doi.org/10.1007/s11101-022-09822-3
Keiper, F., & Atanassova, A. (2022). Enabling Genome Editing for Enhanced Agricultural Sustainability. Frontiers in Genome Editing, 4. https://doi.org/10.3389/fgeed.2022.898950
Khamrit, R., & Jongrungklang, N. (2024). Determining Optimal Mutation Induction of Philodendron billietiae Using Gamma Radiation and In Vitro Tissue Culture Techniques. Horticulturae, 10(11), 1164. https://doi.org/10.3390/horticulturae10111164
Kiani, D., Borzouei, A., Ramezanpour, S., Soltanloo, H., & Saadati, S. (2022). Application of gamma irradiation on morphological, biochemical, and molecular aspects of wheat (Triticum aestivum L.) under different seed moisture contents. Scientific Reports. https://doi.org/10.1038/s41598-022-14949-6
Kumar, D. A. S., & Sankar, P. D. (2024). Plant Bioactive Compounds: Crucial Pharmacological Properties and Role of Elicitors in Enhancing Production. Indian Journal of Pharmaceutical Education and Research, 58(4), 1034. https://doi.org/10.5530/ijper.58.4.115
Merina Chanu, A., Thokchom, R., Bhaigyabati, T., Sandhyarani Devi, N., Ronald Meitei, T., & Bhaigyabati, T. (2020). Effect of gamma irradiation on the chlorophyll content of tree tomato (Solanum betaceum Cav.) in M1 generation. ~ 33 ~ The Pharma Innovation Journal.
Miceli, N., Kwiecień, I., Nicosia, N., Speranza, J., Ragusa, S., Cavò, E., Davì, F., Taviano, M. F., & Ekiert, H. (2023). Improvement in the Biosynthesis of Antioxidant-Active Metabolites in In Vitro Cultures of Isatis tinctoria (Brassicaceae) by Biotechnological Methods/Elicitation and Precursor Feeding. Antioxidants, 12(5), 1111. https://doi.org/10.3390/antiox12051111
Mohanta, T. K., Mishra, A. K., Mohanta, Y. K., & Al-Harrasi, A. (2021). Space Breeding: The Next-Generation Crops [Review of Space Breeding: The Next-Generation Crops]. Frontiers in Plant Science, 12. Frontiers Media. https://doi.org/10.3389/fpls.2021.771985
Muhallilin, I., Aisyah, S. I., & Sukma, D. (2019). The diversity of morphological characteristics and chemical content of celosia Cristata plantlets due to gamma ray irradiation. Biodiversitas. https://doi.org/10.13057/biodiv/d200333
Musa, Y., Sjahril, R., Nadir, M., Khaerani, P. I., & Sakinah, A. I. (2021). Early growth of post-gamma irradiated Indigofera zollingeriana. IOP Conference Series Earth and Environmental Science, 807(3), 32026. https://doi.org/10.1088/1755-1315/807/3/032026
Nandariyah, Yuniastuti, E., Sukaya, & Yudhita, S. I. (2021). Selection for Growth Traits on M1V1 Generation of Raja Bulu Banana (Musa paradisiaca Linn.) Obtained by Gamma Rays Irradiation. Caraka Tani: Journal of Sustainable Agriculture. https://doi.org/10.20961/carakatani.v36i1.34492
Nindyaresmi, A., Umami, N., Kurniawati, A., Anggriani, R., Agussalim, A., & Rahman, M. M. (2025). Mutagenesis Effect Using Gamma Ray Radiation on Morphological Changes, Productivity, and Genetic Variation in Chloris gayana cv. Callide. Pertanika Journal of Tropical Agricultural Science, 48(2). https://doi.org/10.47836/pjtas.48.2.13
Ntsefong, G. N., Ernest, F. P., Constant, L.-L.-N. B., Kinsley, T. M., Hervé, Z. A., Noelle, M. H., & Martin, B. J. (2023). Gamma Ray Induced Mutagenesis for Crop Improvement: Applications, Advancements, and Challenges. In IntechOpen eBooks. IntechOpen. https://doi.org/10.5772/intechopen.1002997
Nur Hanifah, W., Nandariyah, Widiyastuti, Y., & Yunus, A. (2022). Morphology and physiology of Echinacea purpurea in the vegetative phase result of gamma ray irradiation. IOP Conference Series: Earth and Environmental Science. https://doi.org/10.1088/1755-1315/1016/1/012015
Oliveira, A. C. de, & Vanavichit, A. (2023). Editorial: Generating useful genetic variation in crops by induced mutation, volume III. Frontiers in Plant Science, 14. https://doi.org/10.3389/fpls.2023.1301977
Penna, S., & Jain, S. M. (2023). Mutation Breeding for Sustainable Food Production and Climate Resilience. https://doi.org/10.1007/978-981-16-9720-3
Potts, J., Jangra, S., Michael, V. N., & Wu, X. (2023). Speed Breeding for Crop Improvement and Food Security. Crops, 3(4), 276. https://doi.org/10.3390/crops3040025
Reshi, Z. A., Ahmad, W., Лукаткин, А. С., & Javed, S. B. (2023). From Nature to Lab: A Review of Secondary Metabolite Biosynthetic Pathways, Environmental Influences, and In Vitro Approaches [Review of From Nature to Lab: A Review of Secondary Metabolite Biosynthetic Pathways, Environmental Influences, and In Vitro Approaches]. Metabolites, 13(8), 895. Multidisciplinary Digital Publishing Institute. https://doi.org/10.3390/metabo13080895
Riviello-Flores, M. de la L., Cadena-Íñiguez, J., Ruíz-Posadas, L. del M., Arévalo-Galarza, Ma. de L., Castillo‐Juárez, I., Soto‐Hernández, M., & Castillo-Martínez, C. R. (2022). Use of Gamma Radiation for the Genetic Improvement of Underutilized Plant Varieties [Review of Use of Gamma Radiation for the Genetic Improvement of Underutilized Plant Varieties]. Plants, 11(9), 1161. Multidisciplinary Digital Publishing Institute. https://doi.org/10.3390/plants11091161
Rodge, R. R., Rajan, R., Sharma, S., & Singh, T. (2023). Standardization of suitable Gamma irradiation doses 60 Co for mutagenesis in strawberry (Fragaria × annanasa Duch) cv. Chandler. Research Square (Research Square). https://doi.org/10.21203/rs.3.rs-3643574/v1
Saibari, I., Barrijal, S., Mouhib, M., Belkadi, N., & Hamim, A. (2023). Gamma irradiation-induced genetic variability and its effects on the phenotypic and agronomic traits of groundnut (Arachis hypogaeaL.). Frontiers in Genetics, 14. https://doi.org/10.3389/fgene.2023.1124632
Scharff, L. B., Saltenis, V. L. R., Jensen, P. E., Baekelandt, A., Burgess, A. J., Burow, M., Ceriotti, A., Cohan, J., Geu‐Flores, F., Halkier, B. A., Haslam, R. P., Inzé, D., Lankhorst, R. K., Murchie, E. H., Napier, J. A., Nacry, P., Parry, M. A. J., Santino, A., Scarano, A., … Pribil, M. (2021). Prospects to improve the nutritional quality of crops. Food and Energy Security, 11(1). https://doi.org/10.1002/fes3.327
Shabani, M., Alemzadeh, A., Nakhoda, B., Razi, H., Houshmandpanah, Z., & Hildebrand, D. F. (2022). Optimized gamma radiation produces physiological and morphological changes that improve seed yield in wheat. Physiology and Molecular Biology of Plants, 28(8), 1571. https://doi.org/10.1007/s12298-022-01225-0
Sharma, P., Singh, S. P., Iqbal, H. M. N., Parra‐Saldívar, R., Varjani, S., & Tong, Y. W. (2022). Genetic modifications associated with sustainability aspects for sustainable developments [Review of Genetic modifications associated with sustainability aspects for sustainable developments]. Bioengineered, 13(4), 9509. Taylor & Francis. https://doi.org/10.1080/21655979.2022.2061146
Susila, E., Susilowati, A., & Yunus, A. (2019). The morphological diversity of Chrysanthemum resulted from gamma ray irradiation. Biodiversitas. https://doi.org/10.13057/biodiv/d200223
Thakur, M., Bhattacharya, S., Khosla, P. K., & Puri, S. (2018). Improving production of plant secondary metabolites through biotic and abiotic elicitation. Journal of Applied Research on Medicinal and Aromatic Plants, 12, 1. https://doi.org/10.1016/j.jarmap.2018.11.004
Tsugawa, H., Rai, A., Saito, K., & Nakabayashi, R. (2021). Metabolomics and complementary techniques to investigate the plant phytochemical cosmos [Review of Metabolomics and complementary techniques to investigate the plant phytochemical cosmos]. Natural Product Reports, 38(10), 1729. Royal Society of Chemistry. https://doi.org/10.1039/d1np00014d
Wang, X., Ma, R., Cui, D., Cao, Q., Shan, Z., & Jiao, Z. (2017). Physio-biochemical and molecular mechanism underlying the enhanced heavy metal tolerance in highland barley seedlings pre-treated with low-dose gamma irradiation. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-14601-8
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